This contains data and code for the manuscript entitled

Led astray by 16S rRNA: phylogenomics reaffirms the monophyly of Methylobacterium and lack of support for Methylorubrum as a genus

Accepted for publication in the ISMEj https://doi.org/10.1093/ismejo/wraf011

Authors: Alexander B Alleman (University of Idaho) Sergey Stolyar (University of Washington) Christopher J Marx (University of Idaho) Corresponding Author: Jean-Baptiste Leducq (Université Laval)

== If using any part of data or code, please cite the following (in addition to this article)==

• 16s rRNA gene sequence alignment used to produce phylogeny in Figure 1A: this article
• Genomic statistics and core genome-based phylogeny from Figure 1C and Figure 2: data are from Leducq et al. 2022 (https://doi.org/10.1093/gbe/evac123)
• For Figure 3A (phenotype on different carbon sources), references can be retrieved in Table S1
• For Figure 3B (metagenomic data) data are from Lee et al. 2022 (https://doi.org/10.3389/fmicb.2022.849573)
• For Figure S1 (ANI classification of Methylobacteriaceae genomes): this article