Skip to content

Trouble running example 2 - Tree of Life #120

@sierra-smith

Description

@sierra-smith

Hello!

I am attempting to run example 2 in order to recreate the microbial tree of life and I am having trouble downloading the reference genomes.

First, I was having trouble downloading the assembly_summary_genbank.txt file remotely so I manually downloaded it and placed it in my environment.

Then, I tried to rerun phylophlan_get_reference
-g all
-o input_genomes/
-n 1
--verbose 2>&1 | tee logs/phylophlan_get_reference.log

And received the error message:

File "taxa2genomes.txt" present
File "taxa2genomes_cpa201901_up201901.txt.bz2" present
Output folder "input_genomes/" present
File "assembly_summary_genbank.txt" present
Downloading 1 reference genomes for k__Bacteria|p__Actinobacteria|c__Actinobacteria|o__Actinomycetales|f__Actinomycetaceae|g__Actinomyces|s__Actinomyces_radingae
Downloading "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/900/106/055/GCA_900106055.1_IMG-taxon_2630968294_annotated_assembly/GCA_900106055.1_IMG-taxon_2630968294_annotated_assembly_genomic.fna.gz" to "input_genomes/GCA_900106055.fna.gz"
[e] unable to download "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/900/106/055/GCA_900106055.1_IMG-taxon_2630968294_annotated_assembly/GCA_900106055.1_IMG-taxon_2630968294_annotated_assembly_genomic.fna.gz"

Can you please let me know what may be causing the problem? I am running this on MacOS Ventura 13.4.

Thank you!
Sierra

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Type

    No type

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions